project: FAANG
assay type: RNA-seq of total RNA
sample storage: frozen, -70 freezer
sample storage processing: cryopreservation in liquid nitrogen (dead tissue)
sampling to preparation interval: 265.0 weeks
experimental protocol:
https://data.faang.org/api/fire_api/samples/INRA_S (show full text...)(hide...)https://data.faang.org/api/fire_api/samples/INRA_SOP_FRAGENCODE_mRNA-seq_library_preparation_20210111.pdf
extraction protocol:
https://data.faang.org/api/fire_api/experiments/IN (show full text...)(hide...)https://data.faang.org/api/fire_api/experiments/INRA_SOP_FRAGENCODE_RNA_extraction_20210111.pdf
library preparation location:
GeT-PlaGe, 24 Chemin de Borde Rouge, 31326 Castane (show full text...)(hide...)GeT-PlaGe, 24 Chemin de Borde Rouge, 31326 Castanet-Tolosan France
library preparation location longitude: 1.50072 decimal degrees
library preparation location latitude: 43.5279 decimal degrees
library preparation date: 2020-10-21 YYYY-MM-DD
sequencing location:
GeT-PlaGe, 24 Chemin de Borde Rouge, 31326 Castane (show full text...)(hide...)GeT-PlaGe, 24 Chemin de Borde Rouge, 31326 Castanet-Tolosan France
sequencing location longitude: 1.50072 decimal degrees
sequencing location latitude: 43.5279 decimal degrees
sequencing date: 2020-11-17 YYYY-MM-DD
experiment target: RNA
rna preparation 3 adapter ligation protocol:
https://data.faang.org/api/fire_api/samples/INRA_S (show full text...)(hide...)https://data.faang.org/api/fire_api/samples/INRA_SOP_FRAGENCODE_mRNA-seq_library_preparation_20210111.pdf
rna preparation 5 adapter ligation protocol:
https://data.faang.org/api/fire_api/samples/INRA_S (show full text...)(hide...)https://data.faang.org/api/fire_api/samples/INRA_SOP_FRAGENCODE_mRNA-seq_library_preparation_20210111.pdf
library generation pcr product isolation protocol:
https://data.faang.org/api/fire_api/samples/INRA_S (show full text...)(hide...)https://data.faang.org/api/fire_api/samples/INRA_SOP_FRAGENCODE_mRNA-seq_library_preparation_20210111.pdf
preparation reverse transcription protocol:
https://data.faang.org/api/fire_api/samples/INRA_S (show full text...)(hide...)https://data.faang.org/api/fire_api/samples/INRA_SOP_FRAGENCODE_mRNA-seq_library_preparation_20210111.pdf
library generation protocol:
https://data.faang.org/api/fire_api/samples/INRA_S (show full text...)(hide...)https://data.faang.org/api/fire_api/samples/INRA_SOP_FRAGENCODE_mRNA-seq_library_preparation_20210111.pdf
read strand: mate 2 sense
rna purity 260-280 ratio: 2.18
rna purity 260-230 ratio: 2.08
rna integrity number: 9.5